Egg white protein polymer: an affinity matrix for protease enrichment and isolation


Menfaatli E., ZİHNİOĞLU F.

SEPARATION SCIENCE AND TECHNOLOGY, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2021
  • Doi Number: 10.1080/01496395.2021.1887235
  • Title of Journal : SEPARATION SCIENCE AND TECHNOLOGY

Abstract

Egg white was precipitated with acetone and cross-linked with glutaraldehyde. Protease specificity of the polymer was investigated with different types of proteases and non-protease proteins. Protein polymer effectively bound trypsin, papain and the different proteases from pancreatin. SDS-PAGE results and diffusion agar plate tests supported the protease-binding efficiency of the polymer. In conclusion, egg white protein polymer can specifically bind proteases and it is a convenient and cost-effective material for enrichment, isolation, and removal of proteases.