Detection of Heat Shock Protein (DnaK, DnaJ and GrpE) Horizontal Gene Transfers Among Acanthamoeba polyphaga, Acanthamoeba Polyphaga Mimivirus (APMV), Amoeba-Infecting Bacteria and Sputnik Virophage


HAGHİ M., KÖSEOGLU A. E. , KARABOZ İ. , ÜN C.

INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH, cilt.7, ss.1618-1622, 2016 (ESCI İndekslerine Giren Dergi)

  • Cilt numarası: 7 Konu: 4
  • Basım Tarihi: 2016
  • Dergi Adı: INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH
  • Sayfa Sayısı: ss.1618-1622

Özet

Acanthamoeba polyphaga mimivirus (APMV) was isolated in 1992.. It's large size, gram positivity and unique genome features attracted many scientists attention in evolutinary and clinical terms since it's discovery. APMV shares its A. polyphaga host environment with other intracellular bacteria including Campylobacter jejuni, Vibrio cholerae, Legionella pneumophila, Listeria monocytogenes. It has been known that the giant viruses have adopted cellular genes during evolutionary process via horizontal gene transfer (HGT) from bacteria and their eukaryotic host. In this study we aim to detect bacterial Heat shock protein (Dnak, DnaJ and GrpE) genes which are a group of evolutionary conserved proteins that are produced by cellular organisms under stress conditions. As a result of BLAST, significant matches have been found between APMV genome and bacterial genome. Therefore, our results support the adoption of Hsp genes via HGT from bacteria. However, any significant match was not found among APMV, A. polyphaga and Sputnik virophage.