In this study, L-phenylalanine grafted 2-hydroxyethyl methacrylate (Phe-graft-Poly(HEMA)) polymeric nanoparticles (PNPs) were synthesized and their using potential for agricultural purposes was evaluated through the assessment of the PNPs characterization and phytotoxicity on wheat (Triticum aestivum L. cv. Gonen). Characterization of Phe-graft-Poly(HEMA) PNPs was carried out by FT-IR spectroscopy, Zeta-Size analysis and SEM. FT-IR analysis showed L-phenylalanine amino acids incorporation into poly(HEMA) nanoparticles and the particle size was measured about 168 nm by Zeta-Size analysis. SEM images showed spherical shape and narrow distribution of the PNPs. Surface area of the PNP was calculated to be 2723 m(2)g(-1). The phytotoxicity of the PNPs on wheat was explored in seed germination and seedling stage. Treated seedlings maintained high germination rate, fresh-dry weights and shoot-root lengths in early growth period. Leaves of 14-days-old wheat seedlings did not present any decrement on photosynthetic efficiency (Fv/Fm) and pigment contents in comparison to control. Hydrogen peroxide and reactive oxygen species (ROS) induced lipid peroxidation levels were measured similar to control level. These results show that Phe-graft-Poly(HEMA) PNPs may be used as non-phytotoxic hydrophobic carrier system to design unique nanoagrochemicals for different agricultural purposes.