In vitro alpha-Glucosidase and alpha-Amylase Enzyme Inhibitory Effects in Elaeagnus angustifolia Leaves Extracts

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Saltan F. Z. , OKUTUCU B. , Canbay H. S. , Ozel D.

EURASIAN JOURNAL OF ANALYTICAL CHEMISTRY, cilt.12, ss.117-126, 2017 (ESCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 12 Konu: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.12973/ejac.2017.00158a
  • Sayfa Sayıları: ss.117-126


This study was performed to demonstrate alpha-glucosidase and alpha-amylase enzyme inhibitor activities using the methanolic extracts of Elaeagnus angustifolia leaves. Methanolic extracts were prepared by two different extraction methods [accelerated solvent extractor (ASE) and Soxhlet apparatus]. The analytical procedure involved the application of liquid chromatography. Our results showed that the ASE extracts inhibited a-amylase (10-100 mu g/ml) and alpha-glucosidase (10-50 mu g/ml) dose-dependently exhibitedrp inhibitory activities with the extracts [alpha-amylase (40%) and alpha-glucosidase (56%)]. RP-HPLC analyses of the methanolic extracts were detected with vanillic acid and 4-hydroxybenzoic acid as the main phenolic acids in all the ASE extracts. Elaeagnus angustifolia leaf extracts may be attributed to the presence of vanillic acid and 4-hydroxybenzoic acid, there by confirming with its traditional use for the management of diabetes mellitus. We implicate that the anti-diabetic activity through in vitro assessments and support to the folkloric use of this plant for controlling type-2 diabetes mellitus in Turkey.