Disinfection is a critical step in the treatment of a root canal before sealing it with post. This study was to evaluate the effects of various root canal disinfection methods on the bond strength of a glassfibre post to root canal dentine. Forty-eight premolars were studied. The root canals were divided into six groups. Glassfibre posts were cemented with a dual cure composite resin cement following disinfection by using Waterlase laser, Key laser, bioactive glass granules, Tubulicid Plus, NaCl solution (control) and NaOCl solution. Specimens were stored for 8 months. The root part of the tooth was cut and prepared into an hourglass shaped specimen for microtensile bond test. Statistical analysis was performed. Failure types were evaluated and SEM images were taken. The bond strength of fibre posts was significantly affected by the type of disinfection. The highest microtensile bond strength was observed in specimens disinfected by using bioactive glass granules, whereas the lowest bond strength values were recorded with NaOCl irrigation. The bond strength of glassfibre post to root canal dentine was affected by disinfection procedure.