A new pyruvate oxidase biosensor based on 3-mercaptopropionic acid/6-aminocaproic acid modified gold electrode


BAYRAM E., AKYILMAZ E.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.42, ss.418-422, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 42 Konu: 6
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3109/21691401.2013.815626
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.418-422

Özet

In the biosensor construction, 3-mercaptopropionic acid (3-MPA) and 6-aminocaproic acid (6-ACA) were used for forming self-assembled monolayer (SAM) on a gold disc electrode and pyruvate oxidase was immobilized on the modified electrode surface by using glutaraldehyde. Biosensor response is linearly related to pyruvate concentration at 2.5-50 mu M, detection limit is 1.87 mu M and response time of the biosensor is 6 s for differential pulse voltammograms. From the repeatability studies (n = 6) for 30.0 mu M pyruvate revealed that the average value ((x) over bar), standard deviation (S. D) and coefficient of variation (CV %) were calculated to be 31.02 mu M, +/- 0.1914 mu M and 0.62%, respectively.