In this study, probiotic properties of 25 lactic acid bacteria (Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus rhamnosus, Lactobacillus plantarum, Enterococcus faecium, Enterococcus durans and Lactococcus lactis) such as bile salt deconjugation and bile salt resistance levels were compared. Wild type lactic acid bacteria strains were particularly used in this study. The most effective bile salt deconjugation among these bacteria was determined in E. faecium 58K, this was followed by E. durans 42E, Lb. bulgaricus K78, Lb. rhamnosus B-442 and Lb. bulgaricus RF24. Regarding the bile salt resistance, the highest viability was determined in Lc. lactis L21, and Lb. bulgaricus strains were found to be more resistant than S. thermophilus. Additionally, viability was detected in two strains of Lb. rhamnosus, these were followed by E. faecium 58K and E. durans 42E. Regarding bile salt deconjugation, it was concluded that Enterococcus species might be more effective, and regarding the viability in bile salt, Lactococcus lactis L21 was found to be more resistant. The highest hydrophobic activity was detected in S. thermophilus 70S strain, while the lowest was determined in S. thermophilus 82S strain. This result showed that hydrophobic properties of lactic acid bacteria may vary depending on the strain.