Double fluorescence assay via a beta-cyclodextrin containing conjugated polymer as a biomimetic material for cocaine sensing


Arslan M., SENGEL T. Y. , GULER E., GÜMÜŞ Z. P. , ALDEMİR E. , Akbulut H., ...Daha Fazla

POLYMER CHEMISTRY, cilt.8, ss.3333-3340, 2017 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 8 Konu: 21
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1039/c7py00420f
  • Dergi Adı: POLYMER CHEMISTRY
  • Sayfa Sayıları: ss.3333-3340

Özet

A double fluorescence mu-well assay that exploits a novel conjugated polymer containing cyclodextrin (CD) as the key component is reported. For the construction of the cocaine bioassay, poly(p-phenylene) with CD units in the main-chain and poly(ethylene glycol) side chains (PPP-CD-g-PEG) was first prepared by Suzuki coupling polymerization and coated on each well as a biomimetic material. Although the poly-phenylene backbone is responsible for the fluorescence properties without an additional fluorophore, PEG and CD provide water solubility and selective complexation with cocaine, respectively. A cocaine antibody was used as a secondary recognition compound after labelling with quantum dots (QDs). Most notably, we show that the two-color fluorescence nature of the assay facilitates double measurement from the same sample and the described strategy can be adapted to various sensing systems.