According to the data of 2015, Turkey ranks first among the OECD countries in infections resistant to antibiotics. The efforts of
preventing unprescribed antibiotic use as being implemented in Turkey so far since 2016 may have shaped this relationship. The
purpose of the study is to determine knowledge level of the adults living in a rural neighborhood close to Aydın about antibiotics
and antibiotic resistance, and their statuses regarding their use. This cross-sectional study was conducted in Aydın. Data were
collected from 411 participants. 26 questions were used for the questionnaire. Knowledge-attitude level was determined with
average of the number of correct answers given in the survey. Sociodemographic variables and the variables consisting
pharmaceutical use variables were defined as independent variable. Linear regression analysis by SPSS 23.0 was for analysis of
the data. Simple linear regression outputs were shown as Model 1, and outputs of the multiple linear regression analysis were
shown by Model 2. Internal consistency Kuder-Richardson 20 reliability coefficient was calculated. It was determined that
self-medication of antibiotics was too low as a result of restriction of unprescribed antibiotic use by the public. It was understood
that those using prescribed antibiotics and receiving information from physicians had more knowledge about antibiotics. Having
received high school and higher education increased the participants' knowledge-attitude level by 1.88 (1.13-2.64) points, having
lived in a city in childhood increased it by 1.07 (0.32-1.82) points, having used antibiotic within the last one month increased it
by 1.12 (0.30-1.94) points, and the fact the last used antibiotic is prescribed by doctor increased it by 2.99 (1.17-4.81) points.
There may be need for a training program which would generalize the "one health" approach that would provide the opportunity
to guide rational antibiotic use in both humans and animals.