A novel amperometric biosensor based on artichoke (Cynara scolymus L.) tissue homogenate immobilized in gelatin for hydrogen peroxide detection


OZTURK G., ERTAS F. N. , Akyilmaz E. , DINCKAYA E. , TURAL H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.32, no.4, pp.637-645, 2004 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 4
  • Publication Date: 2004
  • Doi Number: 10.1081/labb-200039660
  • Title of Journal : ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Page Numbers: pp.637-645

Abstract

A biosensor for specific determination of hydrogen peroxide was developed by using homogenized artichoke (Cynara scolymus L.) tissue in combination with a dissolved oxygen probe and applied in determination of hydrogen peroxide in milk samples. Artichoke tissue, which has catalase activity, was immobilized with gelatine by means of glutaraldehyde and fixed on a pretreated teflon membrane. The electrode response was maximum when 0.05 M phosphate buffer was used at pH 7.0 and at 30degreesC. Upon addition of hydrogen peroxide, the electrode gives a linear response in a concentration range of 5.0-50x10(-5) M with a response time of 3 min. The method was also applied to the determination of hydrogen peroxide in milk samples.