In Vivo Efficiency of Ex Vivo-Expanded Limbal Epithelial Stem Cells on Functionalised Collagen Type-I Films


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Gürdal M., ERCAN G. , Zeugolis D. I.

The 5th Annual Matrix Biology Ireland, Galway, Ireland, 21 November 2018, pp.53

  • Publication Type: Conference Paper / Full Text
  • City: Galway
  • Country: Ireland
  • Page Numbers: pp.53

Abstract

Limbal epithelilal stem cells (LESCs) in the limbus region of the eye provide lifelong renewal of the corneal epithelium. Damage of the limbus region may cause failure of LESCs activity and as a result, severe vision loss occurs. The most common treatment approach is the transplantation of ex vivo cultured LESCs, which are expanded on the human amniotic membrane (hAM). Despite the positive clinical data to-date, hAM is far from optimal. The aim of this study is to develop an alternative surface material to the hAM for ex vivoexpansion of LESCs and to provide optimum growth conditions. In this study, bovine collagen type I was functionalised with glycosaminoglycans and cross-linked with 4-arm PEG in order to fabricate films. Produced films will be evaluated in rabbit limbal explant culture with a macromolecular crowder (MMC), carrageenan, in terms of their biological efficiency and phenotype maintenance of LESCs in vitro. After in vitro evaluations, ex vivo expanded cells on the optimal film and MMC conditions will be evaluated in limbal stem cell deficiency rabbit model and compared to ex vivo expanded cells on hAM.