Regeneration capacities of five explants (cotyledon, leaf, epicotyl, hypocotyl, apical shoot tip) of bird's-foot trefoil (Lotus corniculatus L.) native race of the Aegean Region in two different basic media (MS and PC-L2), supplemented with different concentrations of 6-benzyladenine (BA), were evaluated using an in vitro system. Shoot tip explants, 3 mg L-1 BA concentration levels, and MS basic media provided the best shoot formation percentage and the maximum number of shoots. The highest shoot length was recorded at shoot tip and epicotyl segments on MS supplemented with 1 mg L-1 BA. To induce rooting, individual elongated shoots were isolated and individually transferred to MS medium containing different concentrations (0.5-2.5 mg L-1) and types of auxins; NAA (alpha-naphthalene acetic acid), IAA (indole-3-acetic acid) and IBA (indole-3-butyric acid). Among different concentrations and types of auxins, the best response for rooting was obtained with 1.0 mg L-1 NAA, considering root formation percentage and root length.