The effect of pretreatment with substrates on the activity of immobilized pancreatic lipase


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Ak G. , Aktuna Y., Kartal F. , Kilinc A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.42, pp.146-150, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42
  • Publication Date: 2014
  • Doi Number: 10.3109/21691401.2013.764312
  • Title of Journal : ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Page Numbers: pp.146-150

Abstract

Porcine pancreatic lipase was covalently immobilized on polyvinyl alcohol using adipoyl dichloride as a cross-linker.The eff ect of pre-treatment of lipase previously with various
types of oils on immobilization effi ciency was investigated. The increment in immobilization efficiency was observed after pre-treatment of lipases with oils. The highest immobilization efficiency obtained was 20% (v/v) for olive oil pre-treated lipase, which was 8 times higher than that of non-pretreated immobilized lipase. Immobilized lipase had better stability and had some advantages in comparison with free enzyme.

Porcine pancreatic lipase was covalently immobilized on polyvinyl alcohol using adipoyl dichloride as a cross-linker. The effect of pre-treatment of lipase previously with various types of oils on immobilization efficiency was investigated. The increment in immobilization efficiency was observed after pre-treatment of lipases with oils. The highest immobilization efficiency obtained was 20% (v/v) for olive oil pre-treated lipase, which was 8 times higher than that of non-pretreated immobilized lipase. Immobilized lipase had better stability and had some advantages in comparison with free enzyme.