Investigation of in vitro efficiency of magnetic nanoparticle-conjugated I-125-uracil glucuronides in adenocarcinoma cells


MEDİNE E. İ. , ÜNAK P. , Sakarya S., Ozkaya F.

JOURNAL OF NANOPARTICLE RESEARCH, vol.13, no.10, pp.4703-4715, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 10
  • Publication Date: 2011
  • Doi Number: 10.1007/s11051-011-0436-6
  • Title of Journal : JOURNAL OF NANOPARTICLE RESEARCH
  • Page Numbers: pp.4703-4715

Abstract

Modification of the magnetic properties of a drug can be used to direct the drug to the desired site, enhancing its therapeutic effectiveness and reducing side effects. In this study, surface-modified magnetic nanoparticles were immobilized with uracil glucuronide derivatives and then labeled with I-125. The morphology, structure, and composition of the magnetic particles were examined by TEM, SEM, VSM, and XRD. The particles sizes were about 50 nm. The labeling yield was 93.8% for uracil-O-glucuronide-immobilized magnetic particles and 95.0% for uracil-N-glucuronide-immobilized magnetic particles. The cell incorporation rates of N- and O-glucuronides were higher than those of uracil. The incorporation rates of uracil-, O-glucuronide-, and N-glucuronide-conjugated magnetic particles were all high. The cell incorporation rates of ligand-conjugated magnetic particles increased under a magnetic field.