DETERMINATION OF FETAL RHESUS D STATUS BY MATERNAL PLASMA DNA ANALYSIS


AYKUT A. , ONAY H. , SAĞOL S. , GÜNDÜZ C. , ÖZKINAY F. F. , Cogulu O.

BALKAN JOURNAL OF MEDICAL GENETICS, cilt.16, ss.33-38, 2013 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 16 Konu: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.2478/bjmg-2013-0029
  • Dergi Adı: BALKAN JOURNAL OF MEDICAL GENETICS
  • Sayfa Sayısı: ss.33-38

Özet

In this study, we assessed the feasibility of fetal RhD genotyping by analysis of cell-free fetal DNA(cffDNA) extracted from plasma samples of Rhesus (Rh) D-negative pregnant women by using real-time polymerase chain reaction (PCR). Fetal genotyping was performed on 30 RhD-negative women between 9 and 39 weeks of gestation who were referred to us for invasive testing [amniocentesis/chorionic villi sampling (CVS)]. The fetal RHD genotype was determined based on real-time PCR method. Exons 7 and 10 of the RHD and SRY genes were targeted.