Bismuth Film Electrode as Sensing Platform for IgE-anti-IgE Interactions


Anik U., Cevik S., TİMUR S.

ELECTROANALYSIS, cilt.23, ss.2379-2385, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 23 Konu: 10
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1002/elan.201100047
  • Dergi Adı: ELECTROANALYSIS
  • Sayfa Sayıları: ss.2379-2385

Özet

In this study, a bismuth film was used as a sensing platform for immunoreaction assay which includes the interactions between IgE and anti-IgE molecules. For preparing this assay, IgE was deposited on a carbon paste electrode via bismuth(III) cations without using any membrane or functional reagent. The immobilized reagents and interaction of anti-IgE on this formation were monitored by using atomic force microscopy technique and electrochemical impedance spectroscopy. Then some experimental parameters like the effect of IgE amount and the IgE anti-IgE interaction time were optimized. Under the optimal experimental conditions, the binding of anti-IgE was also examined by following current decrease in differential pulse voltammograms of neutral red.