Bismuth Film Electrode as Sensing Platform for IgE-anti-IgE Interactions


Anik U., Cevik S., TİMUR S.

ELECTROANALYSIS, vol.23, no.10, pp.2379-2385, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 10
  • Publication Date: 2011
  • Doi Number: 10.1002/elan.201100047
  • Title of Journal : ELECTROANALYSIS
  • Page Numbers: pp.2379-2385

Abstract

In this study, a bismuth film was used as a sensing platform for immunoreaction assay which includes the interactions between IgE and anti-IgE molecules. For preparing this assay, IgE was deposited on a carbon paste electrode via bismuth(III) cations without using any membrane or functional reagent. The immobilized reagents and interaction of anti-IgE on this formation were monitored by using atomic force microscopy technique and electrochemical impedance spectroscopy. Then some experimental parameters like the effect of IgE amount and the IgE anti-IgE interaction time were optimized. Under the optimal experimental conditions, the binding of anti-IgE was also examined by following current decrease in differential pulse voltammograms of neutral red.