A Ready-to-Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphisms

KAYRAN Y. U. , Ozkan-Ariksoysal D. , Tezcanli B. , KOSOVA B. , OZSOZ M.

ELECTROANALYSIS, cilt.25, ss.2668-2676, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 25 Konu: 12
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/elan.201300386
  • Dergi Adı: ELECTROANALYSIS
  • Sayfa Sayısı: ss.2668-2676

Özet

In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol-O-methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a kit and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18pmol of synthetic target sequence and 6.09x10(5) molecules of real samples in 30min detection time.