ELECTROANALYSIS, cilt.25, ss.2668-2676, 2013 (SCI İndekslerine Giren Dergi)
In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol-O-methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a kit and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18pmol of synthetic target sequence and 6.09x10(5) molecules of real samples in 30min detection time.