Exogenous glycinebetaine affects growth and proline accumulation and retards senescence in two rice cultivars under NaCl stress

DEMİRAL T., Turkan I.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, vol.56, no.1, pp.72-79, 2006 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 1
  • Publication Date: 2006
  • Doi Number: 10.1016/j.envexpbot.2005.01.005
  • Page Numbers: pp.72-79


The ability of exogenously applied glycinebetaine (GB) for the alleviation of growth inhibition and senescence resulting from NaCl stress was investigated in view of growth parameters, net PS-II efficiency, photosynthetic pigments, protein and proline contents in two rice (Oryza sativa L.) cultivars differing in salt tolerance (salt-tolerant Pokkali, and salt-sensitive IR-28). Shoot fresh weight (FW) of Pokkali and shoot and root dry weight of IR-28 showed a decrease under salinity but an increase with exogenous GB application. NaCl treatment caused no serious decrease in F-V/F-M ratio of Pokkali whereas F-V/F-M ratio of IR-28 showed a remarkable decline throughout the experiment. Exogenous GB application partially preserved net PS-II efficiency of IR-28 from salt-stress. While ChI-b and carotenoid contents of IR-28 decreased, photosynthetic pigment contents of Pokkali showed no notable change under salinity. GB treatment did not show protective effect on photosynthetic pigment contents of IR-28 decreased under-salinity. Proline content significantly increased in Pokkali and IR-28 under salt-stress with a higher rate of increase in the former. When compared to NaCl-exposed groups alone, GB treatment caused an increase and a decrease in the level of proline accumulation in Pokkali and IR-28, respectively. While protein content of Pokkali enhanced with salinity, GB pre-application decreased it to control levels. Protein content of IR-28 showed a decrease under salinity but an increase with GB application. Therefore, we conclude that GB can reduce the impact of salt-stress on growth and senescence, enhance the photosynthetic efficiency of PS-II and increase the protein content of rice seedlings. (c) 2005 Elsevier B.V. All rights reserved.