Photophysical characterization of fluorescent oxazol-5-one derivatives in PVC and their application as biosensors in the detection of ACh and AChE inhibitor: Donepezil


ÖZTÜRK G., ALP S., Timur S.

DYES AND PIGMENTS, vol.76, no.3, pp.792-798, 2008 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 76 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.1016/j.dyepig.2007.02.005
  • Title of Journal : DYES AND PIGMENTS
  • Page Numbers: pp.792-798
  • Keywords: acetylcholinesterase, neurotransmitter, donepezil, 5-oxazolone, fluorescence emission, biosensor, IMMOBILIZED ACETYLCHOLINESTERASE, ORGANOPHOSPHORUS INSECTICIDES, AMPEROMETRIC BIOSENSOR, CARBAMATE PESTICIDES, FIBEROPTIC BIOSENSOR, CHOLINE OXIDASE, ELECTRODES, COLUMN, HPLC, HYDROCHLORIDE

Abstract

Reversible biosensors based on 2-(3,5 -dinitrophenyl)-4-[4-(1,4,7, 10-tetraoxa-13-azacyclopentadecyl) benzylidene]-5-oxazolone (CPO-I), 2-(4-nitrophenyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidenej-5-oxazolone (CPO-II) and 2-(4-tolyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzy lidene]-5-oxazol one (CPO-III) derivatives immobilized in a polyvinylchloride matrix, doped with acetylcholinesterase, have been developed. The biosensors were calibrated for acetylcholine as neurotransmitter and the potent, selective and reversible cholinesterase inhibitor, donepezil ((+/-)-2-[(1-benzyl-piperidine-4-yl)ethyl]-5,6-dimetoxyindan-1-one hydrochloride). The photophysical parameters of all derivatives were also examined in PVC in which medium, they exhibited excellent photostability. (c) 2007 Elsevier Ltd. All rights reserved.