Use of Eggshell Membrane as an Immobilization Platform in Microbial Sensing

YENİ F., Odaci D. , TİMUR S.

ANALYTICAL LETTERS, cilt.41, ss.2743-2758, 2008 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 41 Konu: 14
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/00032710802363594
  • Sayfa Sayıları: ss.2743-2758


A new microbial biosensor was developed by immobilizing Pseudomonas fluorescens cells on eggshell membrane via physical adsorbtion. Bacteria-modified eggshell membrane was fixed tightly onto the surface of a carbon paste electrode (CPE) with a silicone rubber o-ring to construct a microbial biosensor. The measurements were based on the respiratory activity of the cells caused by oxygen consumption during bacterial metabolism. A mediated biosensor was also developed by ferrocene. As well as the response characteristics, stabilities and substrate specificities were investigated. Data were given as the comparison of mediated and unmediated systems.