Use of Eggshell Membrane as an Immobilization Platform in Microbial Sensing


YENİ F., Odaci D. , TİMUR S.

ANALYTICAL LETTERS, vol.41, no.14, pp.2743-2758, 2008 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 14
  • Publication Date: 2008
  • Doi Number: 10.1080/00032710802363594
  • Title of Journal : ANALYTICAL LETTERS
  • Page Numbers: pp.2743-2758
  • Keywords: Eggshell membrane, mediator, microbial sensor, pseudomonas fluorescens, ENZYME IMMOBILIZATION, GLUCOSE-OXIDASE, PSEUDOMONAS-PUTIDA, OPTICAL BIOSENSOR, COLLAGEN MATRIX, SHELL MEMBRANE, EGG-SHELL, PROTEIN, ELECTRODES, VEGETABLES

Abstract

A new microbial biosensor was developed by immobilizing Pseudomonas fluorescens cells on eggshell membrane via physical adsorbtion. Bacteria-modified eggshell membrane was fixed tightly onto the surface of a carbon paste electrode (CPE) with a silicone rubber o-ring to construct a microbial biosensor. The measurements were based on the respiratory activity of the cells caused by oxygen consumption during bacterial metabolism. A mediated biosensor was also developed by ferrocene. As well as the response characteristics, stabilities and substrate specificities were investigated. Data were given as the comparison of mediated and unmediated systems.