Construction of a multi-epitope vaccine candidate against <i>Fasciola hepatica</i>: an <i>in silico</i> design using various immunogenic excretory/secretory antigens.


Akil M., Aykur M., Karakavuk M., Can H. , Döşkaya M.

Expert review of vaccines, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2021
  • Doi Number: 10.1080/14760584.2022.1996233
  • Title of Journal : Expert review of vaccines
  • Keywords: Fasciola hepatica, excretory, secretory (E, S) antigens, in silico analysis, multiepitope vaccine, vaccine development, MULTI-EPITOPE VACCINE, B-CELL EPITOPES, PROTEIN-STRUCTURE, PEPTIDE VACCINE, PREDICTION, ACTIVATION, INHIBITOR, INFECTION, ANTIBODY, REGION

Abstract

Background Fasciola hepatica is an important pathogen that causes liver fluke disease in definitive hosts such as livestock animals and humans. Various excretory/secretory products have been used in serological diagnosis and vaccination studies targeting fasciolosis. There are no commercial vaccines against fasciolosis yet. Bioinformatic analysis based on computational methods have lower cost and provide faster output compared to conventional vaccine antigen discovery techniques. The aim of this study was to predict B- and T-cell specific epitopes of four excretory/secretory antigens (Kunitz-type serine protease inhibitor, cathepsin L1, helminth defense molecule, and glutathione S-transferase) of Fasciola hepatica and to construct a multiepitope vaccine candidate against fasciolosis. Methods and Results Initially, nonallergic and the highest antigenic B- and T- cell epitopes were selected and then, physico-chemical parameters, secondary and tertiary structures of designed multiepitope vaccine candidate were predicted. Tertiary structure was refined and validated using online bioinformatic tools. Linear and discontinuous B-cell epitopes and disulfide bonds were determined. Finally, molecular docking analysis for MHC-I and MHC-II receptors was performed. Conclusion This multi-epitope vaccine candidate antigen, with high immunological properties, can be considered as a promising vaccine candidate for animal experiments and wet lab studies.