Polyclonal Antibody Production Against Hapten-Structured KDN Molecule by Using Different Adjuvants Alternative to Freund's Adjuvant


Iz S. , Metiner P. S. , Kimiz I., Kayali C., Gurhan S. I. D.

EUROPEAN JOURNAL OF THERAPEUTICS, vol.24, no.2, pp.106-111, 2018 (Journal Indexed in ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.5152/eurjther.2018.400
  • Title of Journal : EUROPEAN JOURNAL OF THERAPEUTICS
  • Page Numbers: pp.106-111

Abstract

Objective: KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid), a member of the sialic acid family, is a hapten-structured low-molecular-weight monosaccharide on the cell membrane, which cannot induce immune responses without a carrier protein. Since it is over-expressed on the cancerous cells' membrane, it is thought to be a great target molecule for anti-cancer treatments. The aim of this study is to obtain high titersof the anti-KDN polyclonal antibody response without using any carrier protein against the hapten-structured KDN molecule alternative to Freund's adjuvants.