Lectin attached affinity cryogels for amyloglucosidase adsorption

Bayraktaroglu M., Orhan H., Evli S., AKGÖL S. , Uygun D. A. , Uygun M.

JOURNAL OF CARBOHYDRATE CHEMISTRY, cilt.37, ss.302-317, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 37 Konu: 5
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/07328303.2018.1487972
  • Sayfa Sayıları: ss.302-317


Preparation of poly[ethylene glycol dimethacrylate (EGDMA)-glycidyl methacrylate (GMA)] cryogel and its usability for amyloglucosidase adsorption were investigated. Cryogels were prepared by cryocopolymerization technique and then functionalized by lectin concanavalin A (Con A). The synthesized cryogel was characterized by FTIR, SEM and EDX analysis and then used for adsorption of amyloglucosidase in a continuous flow system. The maximal amyloglucosidase adsorption efficiency of Con A attached cryogel was found to be as 30.50mg/g cryogel. Con A modified cryogels were used repeatedly for 30 times without any significant decrease at the amyloglucosidase adsorption capacity. SDS-PAGE and activity studies confirmed that the desorbed amyloglucosidase was active and retained 90% of its initial activity after the adsorption/desorption processes.