Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design


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ECE S., EVRAN S. , JANDA J., MERKL R., STERNER R.

PROTEIN ENGINEERING DESIGN & SELECTION, cilt.28, ss.147-151, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 28 Konu: 6
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1093/protein/gzv001
  • Dergi Adı: PROTEIN ENGINEERING DESIGN & SELECTION
  • Sayfa Sayıları: ss.147-151

Özet

Neopullulanase, a glycosyl hydrolase from Bacillus stearothermophilus (bsNpl), is a potentially valuable enzyme for starch and detergent industries. However, as the protein is not active at elevated temperatures and high surfactant concentrations, we aimed to increase its stability by rational enzyme design. Nine potentially destabilizing cavities were identified in the crystal structure of the enzyme. Based on computational predictions, these cavities were filled by residues with bulkier side chains. The five Asp46Glu, Val239Leu, Val404Leu, Ser407Thr and Ala566Leu exchanges resulted in a drastic stabilization of bsNpl against inactivation by heat and detergents. The catalytic activity of the variants was identical to the wild-type enzyme.