Calixarene modified montmorillonite: a novel design for biosensing applications

SONMEZ B., Sayin S., YALÇINKAYA E. E. , SELECI D. A. , Yildiz H. B. , Demirkol D. , ...More

RSC ADVANCES, vol.4, no.108, pp.62895-62902, 2014 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 108
  • Publication Date: 2014
  • Doi Number: 10.1039/c4ra11818a
  • Title of Journal : RSC ADVANCES
  • Page Numbers: pp.62895-62902


Here we report the synthesis, characterization and application of calixarene (Calix) modified montmorillonite (Mt) as a platform for bio-applications such as biomolecule immobilization and biosensing technologies. This modification enhanced the biomolecule immobilization capability of Mt. Initially, amino-functionalised calixarenes (Calix-NH2) were synthesized and used as a modifier. X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric analysis were performed to verify the modification of the clay minerals. For the biosensor construction, Calix-NH2 modified Mt (Calix-NH2/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and pyranose oxidase were immobilized on the surface of a glassy carbon electrode which was then referred to as a CalixNH(2)/ Mt/PyOx biosensor. After optimization of the enzyme amount and pH, analytical characteristics were investigated in detail.