New Phytologist Next Generation Scientist, Dublin, İrlanda, 22 - 25 Temmuz 2019, ss.105
Accumulation of unfolded
proteins in ER is called ER stress. In response to ER stress, the unfolded
protein response (UPR) is triggered to increase the protein folding capacity.
ER stress can also be induced with agents such as tunicamycin (Tm). Proline is
a well-known amino acid that takes vital roles under environmental stresses. Previously, it has been observed that yeast strains
deficient in proline biosynthesis were sensitive to ER stress. The aim of the
study is to determine the effects of Tm induced ER stress at level of gene
expression and enzyme activities of proteins related to proline biosynthesis
and catabolism and to compare the ER stress tolerance of different A. thaliana genotypes that have low (p5cs1-1) and high (prodh2-2, prodh1-4, R2M12-4) endogenous proline levels to ER
stress. For this aim, the effects of ER stress on these mutants were evaluated
by phenotyping their root growth. Moreover, the responses to ER stress of these
mutants were determined by measuring the transcript abundance of ER stress
related genes by qRT-PCR. p5cs1-1 mutant
was more sensitive to ER stress and showed lower induction of UPR genes while
the opposite was observed in genotypes with high proline levels.