Interplay between proline and unfolded protein response in endoplasmic reticulum stress tolerance of Arabidopsis thaliana


Özgür Uzilday R. , Uzilday B. , Türkan İ. , Szabados L.

New Phytologist Next Generation Scientist, Dublin, İrlanda, 22 - 25 Temmuz 2019, ss.105

  • Basıldığı Şehir: Dublin
  • Basıldığı Ülke: İrlanda
  • Sayfa Sayıları: ss.105

Özet

Accumulation of unfolded proteins in ER is called ER stress. In response to ER stress, the unfolded protein response (UPR) is triggered to increase the protein folding capacity. ER stress can also be induced with agents such as tunicamycin (Tm). Proline is a well-known amino acid that takes vital roles under environmental stresses. Previously, it has been observed that yeast strains deficient in proline biosynthesis were sensitive to ER stress. The aim of the study is to determine the effects of Tm induced ER stress at level of gene expression and enzyme activities of proteins related to proline biosynthesis and catabolism and to compare the ER stress tolerance of different A. thaliana genotypes that have low (p5cs1-1) and high (prodh2-2, prodh1-4, R2M12-4) endogenous proline levels to ER stress. For this aim, the effects of ER stress on these mutants were evaluated by phenotyping their root growth. Moreover, the responses to ER stress of these mutants were determined by measuring the transcript abundance of ER stress related genes by qRT-PCR. p5cs1-1 mutant was more sensitive to ER stress and showed lower induction of UPR genes while the opposite was observed in genotypes with high proline levels.