The effects of egg storage duration (ESD) and brooding temperature (BT) on BW, intestine development and nutrient transporters of broiler chicks were investigated. A total of 396 chicks obtained from eggs stored at 18 degrees C for 3 days (ESD3-18 degrees C) or at 14 degrees C for 14 days (ESD14-14 degrees C) before incubation were exposed to three BTs. Temperatures were initially set at 32 degrees C, 34 degrees C and 30 degrees C for control (BT-Cont), high (BT-High) and low (BT-Low) BTs, respectively. Brooding temperatures were decreased by 2 degrees C each at days 2, 7, 14 and 21. Body weight was measured at the day of hatch, 2, 7, 14, 21, 28 and 42. Cloacal temperatures of broilers were recorded from 1 to 14 days. Intestinal morphology and gene expression levels of H+-dependent peptide transporter (PepT1) and Na-dependent glucose (SGLT1) were evaluated on the day of hatch and 14. Cloacal temperatures of chicks were affected by BTs from days 1 to 8, being the lowest for BT-Low chicks. BT-High resulted in the heaviest BWs at 7 days, especially for ESD14-14 degrees C chicks. This result was consistent with longer villus and larger villus area of ESD14-14 degrees C chicks at BT-High conditions. From 14 days to slaughter age, BT had no effect on broiler weight. ESD3-18 degrees C chicks were heavier than ESD14-14 degrees C chicks up to 28 days. The PepT1 and SGLT1 expression levels were significantly higher in ESD3-18 degrees C chicks than ESD14-14 degrees C on the day of hatch. There was significant egg storage by BT interaction for PepT1 and SGLT1 transporters at day 14. ESD14-14 degrees C chicks had significantly higher expression of PepT1 and SGLT1 at BT-Low than those at BT-Cont. ESD14-14 degrees C chicks upregulated PepT1 gene expression 1.15 and 1.57-fold at BT-High and BT-Low, respectively, compared with BT-Cont, whereas PepT1 expression was downregulated 0.67 and 0.62-fold in ESD3-18 degrees C chicks at BT-High and BT-Low. These results indicated that pre-incubation egg storage conditions and BTs affected intestine morphology and PepT1 and SGLT1 nutrient transporters expression in broiler chicks.