The enhancement of polygalacturonase and polymethylgalacturonase production on solid-state conditions by Aspergillus foetidus


Taskin E., ELTEM R.

FOOD BIOTECHNOLOGY, vol.22, no.3, pp.203-217, 2008 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.1080/08905430802262533
  • Title of Journal : FOOD BIOTECHNOLOGY
  • Page Numbers: pp.203-217
  • Keywords: enhancement of pectinase, polygalacturonase, polymethylgalacturonase, solid state, sugar beet pulp, Aspergillus foetidus Ege-K-635, SUGAR-BEET PULP, PECTOLYTIC ENZYMES, AGROINDUSTRIAL RESIDUES, PECTINASE PRODUCTION, APPLE POMACE, NIGER, FERMENTATION, WASTE, OPTIMIZATION, PARAMETERS

Abstract

In this study, a previously isolated strain of Aspergillus foetidus Ege-K-635 producing highly active polygalacturonase and polymethylgalacturonase was used for improvement of enzyme production using solid-state conditions. To enhance the enzyme yield, the mixture of two substrates such as sugar beet pulp and wheat bran, having different ratios of carbon-nitrogen and moisture levels was used. Further the effect of the amount of inoculum was investigated. Physical parameters for the highest polygalacturonase and polymethylgalacturonase production under solid state conditions were determined on wheat bran: the sugar beet pulp (1:2) mixture substrate composition having a 31:1 carbon-nitrogen ratio with 8 x 10(6) spores/gram of dry substrate as the inoculum amount. Adequate moisture levels were 75% and 70% for polygalacturonase and polymethylgalacturonase, respectively. Under these conditions, the highest activities obtained were 385 +/- 12.3 Ug(-1) on the third day for polygalacturonase and 18.3 +/- 3.2 Ug(-1) on the second day for polymethylgalacturonase.