The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid


Karadeniz H. , Armagan G. , Erdem A. , Turunc E., Çalışkan A., Kanıt L. , ...Daha Fazla

ELECTROANALYSIS, cilt.21, ss.2468-2476, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 21
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1002/elan.200900218
  • Dergi Adı: ELECTROANALYSIS
  • Sayfa Sayıları: ss.2468-2476

Özet

A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded DNA and genomic DNA was herein determined in in vitro and in vivo conditions using; electrochemical assay and agarose gel electrophoresis. The changes in guanine signal were detected as an indicator of DNA damage in genomic DNA samples isolated from 1 or 10 mg/kg KA-treated animals. The decreased levels of guanine signal were found as 29% and 33% by 1 and 10 mg/kg KA treatment when compared to controls, respectively. The results of gel electrophoresis confirmed DNA damage obtained in identical samples by electrochemical method.