In vitro evaluation of radiolabeled (I-125) methanol extracts of yarrow in cell lines of MCF-7, PC-3, A-549 and Caco-2


Kilcar A. , ÇEKİÇ B., MUFTULER F. Z. B. , ÜNAK P. , MEDİNE E. İ.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.295, ss.593-599, 2013 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 295 Konu: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1007/s10967-012-1895-7
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Sayfa Sayısı: ss.593-599

Özet

Nowadays, cancer is still the second leading cause of death all over the world. Therefore, natural products which have anticancer and antitumor properties are come into prominence. Achillea family is known with anticancer and antitumor activity. Yarrow which has over a hundred bioactive compounds is a member of Achillea family. In current study; components of yarrow which was obtained after methanol extraction and purification were radiolabeled with I-125 and effects of these radiolabeled components on the cells were examined with using Caco-2, MCF-7, A-549, PC-3 cell lines. As a result of these studies, seven peaks were obtained and the highest radiolabeling yield was calculated for I-125 radiolabeled Peak 7 (95.00 +/- A 7.07, n = 4). To screen the biological properties of these radiolabeled peaks at determined cell lines, our ongoing effort was to evaluate incorporation percentage with time dependent. Furthermore, I-125-Peak 7 had highest incorporation ratio for whole cell lines and its incorporation percentage was increased with time dependent. Results of these in vitro studies were compatible with previous in vivo studies and traditional use of yarrow plants.