EVALUATON OF POTANTIAL HARMFUL EFFECTS OF POLYETHYLENE MICROPLASTICS IN FISH DIGESTIVE SYSTEM A LABORATORY MODEL STUDY WITH ZEBRAFISH


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Gökçe B., İşisağ Üçüncü S.

International Marmara Sciences Congress (Autumn) 2019, Kocaeli, Türkiye, 1 - 03 Kasım 2019, ss.1179-1183

  • Basıldığı Şehir: Kocaeli
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1179-1183

Özet

Plastic materials are probably the most multifaceted synthetic materials invented by humans. They are widely used because of their unique properties such as weight, strength, durability, corrosion resistance and electrical insulation. In the last 50 years, the annual world production of plastics increased drastically. In 2012 280 million tons of plastics were produced globally and half of them disposed in landfills or recycled. However they are also discarded to the environment as a plastic litter. In the environment, larger plastic items can slowly breakdown into small pieces via physical and chemicals factors. Plastic debris under 5 mm diameter is usually called as microplastic and they can be ingested by aquatic organisms and cause potentially harmful effects. Polyethylene (PE) is one of the plastic polymer with the highest global production and the most identified in marine litter. PE has a very love glass transition temperature and plasticizers are not needed in PE objects. However, it has low durability under environmental conditions and easily breaks down into small particles. In our study potential, the harmful effects of PE microparticles on the zebrafish digestive system were evaluated. Four different concentrations (0.05, 0.1, 0.2 and 0.4 ppm) of PE microparticles were applied to male zebrafish specimens during 96 hours. At the end of exposure duration, animals were dissected and digestive system tissues were fixed with bouin. Tissues were embedded in paraffin blocks, sectioned and stained. They were examined with light microscopy and histopathological changes in tissue sections were reported.