Egg white hybrid nanoflower (EW-hNF) with biomimetic polyphenol oxidase reactivity: Synthesis, characterization and potential use in decolorization of synthetic dyes


Altinkaynak C., Kocazorbaz E. , ÖZDEMİR N., ZİHNİOĞLU F.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.109, pp.205-211, 2018 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 109
  • Publication Date: 2018
  • Doi Number: 10.1016/j.ijbiomac.2017.12.072
  • Title of Journal : INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Page Numbers: pp.205-211
  • Keywords: Enzyme mimic, Hybrid nanoflowers, Egg white, Cu(II) ion, TEXTILE DYES, PEROXIDASE, NANOPARTICLES, NANOMATERIALS, STABILITY, IMMOBILIZATION, TECHNOLOGIES, ENZYMES, STRAIN, SENSOR

Abstract

In this study, for the first time, we described organic-inorganic hybrid nanoflowers using crude egg white as the organic component and copper (II) ions as the inorganic component under the mild conditions. The synthesized egg white-inorganic hybrid nanoflowers (EW-hNFs) were characterized using SEM, EDX, XRD and FTIR analysis. The biomimetic Polyphenol/Peroxidase like activities of synthesized egg white inorganic hybrid nanoflowers (EW-hNFs) were determined by using various phenolics with or without H2O2. Optimum pH and temperature, kinetic parameters, reusability, pH and thermal stability of EW-hNFs were also studied. The most noteworthy aspect of our study is that synthesized EW-hNFs which consist of only egg white proteins, showed polyphenol oxidase activity. Furthermore, potential use of the EW-hNFs in the discoloration of the some synthetic dyes was also evaluated. (C) 2017 Elsevier B.V. All rights reserved.