The dorsal and ventral skin of the Eastern tree frog, Hyla orientalis, was investigated by light microscopy. Histochemistry was carried out with use of various staining methods, and microscopic observations revealed the morphological characteristics of the skin with squamous epithelium in the epidermis and connective tissue in the dermis, subdivided into stratum spongiosum and stratum compactum. Two types of skin glands (mucous and granular) were observed in both dorsal and ventral skin. The percentage of dermal glands was calculated and diameters of granular and mucous glands were measured per animal in, both dorsal and ventral regions. The parameters were compared by one-way ANOVA and subsequently by Mann Whitney U-test in terms of statistical differences in the diameters of dermal glands. The histochemical properties and morphological characteristics with qualitative and quantitative features for each region (dorsal and ventral integuments) did not show individual variation and may be considered as important features for taxonomic, phylogenetic and ecological studies.