Protective clothing protects the body from external influence like heat, chemicals, mechanical hazards, bad weather, etc. by shielding the human body from harsh environmental effects. The maintenance of thermal balance is one of the most important aspects of protective clothing. The study aims to investigate the thermal comfort properties of woven hybrid fabrics produced with high performance core spun yarns. For this purpose, meta-aramid, e-glass, Technora (R) and Dyneema (R) fibres were combined using core yarn spinning method in order to enhance the protective performance characteristics. The effects of the core/sheath ratio and type of core materials on the thermal comfort characteristics were investigated and evaluated statistically. The results revealed that, core/sheath ratio, types of core materials have significant effects on thermal comfort characteristics of the fabrics. As the core ratio increases from 19 to 56%, the air permeability of the fabrics increases whereas their thermal conductivity and thermal absorptivity properties decrease. Meta-aramid/E-glass core fabrics can be preferable for protective clothing due to high air permeability and thermal conductivity values.