Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection


Erdem A. , CONGUR G.

ELECTROANALYSIS, vol.30, no.1, pp.67-74, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.1002/elan.201700462
  • Title of Journal : ELECTROANALYSIS
  • Page Numbers: pp.67-74
  • Keywords: Hydroxyapatite nanoparticles (HaNPs), pencil graphite electrode, electrochemical biosensor, differential pulse voltammetry (DPV), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), REDUCED GRAPHENE OXIDE, CARBON NANOTUBES, IMPEDIMETRIC DETECTION, ACID HYBRIDIZATION, PENCIL ELECTRODE, BIOSENSOR, FABRICATION, SENSOR, IMMOBILIZATION

Abstract

Hydroxyapatite nanoparticles (HaNP) modified pencil graphite electrodes (PGEs) were developed for the first time in the literature, and accordingly they were applied for electrochemical monitoring of sequence-selective DNA hybridization. The experimental conditions for HaNP modification of PGE, and DNA hybridization related to Hepatitis B Virus (HBV) DNA sequence were optimized. The microscopic and electrochemical characterization of HaNP-PGE in contrast to the unmodified one was utilized. Under optimized experimental conditions, the selectivity of HBV DNA probe immobilized biosensor was tested against to non-complementary (NC), mismatch (MM) sequences and the mixture of target:NC (1:1) or target: MM (1:1).