Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection


Erdem A. , CONGUR G.

ELECTROANALYSIS, cilt.30, ss.67-74, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 30 Konu: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/elan.201700462
  • Dergi Adı: ELECTROANALYSIS
  • Sayfa Sayıları: ss.67-74

Özet

Hydroxyapatite nanoparticles (HaNP) modified pencil graphite electrodes (PGEs) were developed for the first time in the literature, and accordingly they were applied for electrochemical monitoring of sequence-selective DNA hybridization. The experimental conditions for HaNP modification of PGE, and DNA hybridization related to Hepatitis B Virus (HBV) DNA sequence were optimized. The microscopic and electrochemical characterization of HaNP-PGE in contrast to the unmodified one was utilized. Under optimized experimental conditions, the selectivity of HBV DNA probe immobilized biosensor was tested against to non-complementary (NC), mismatch (MM) sequences and the mixture of target:NC (1:1) or target: MM (1:1).