There are various diagnosis and treatment monitoring are available for many neuro-muscular diseases. This paper is concerned with the tremor analysis for the purpose of recognition. A recording and analysis system for human's tremor are developed. The analysis was performed based on frequency and amplitude parameters. The fast Fourier transform (FFT) and higher-order spectra were used to extract frequency parameters (e.g., main peak frequency). In order to diagnose subjects' condition, classification was implemented by statistical significant tests (t-test). (c) 2006 Elsevier Ltd. All rights reserved.