Production of Monoclonal Antibody Against Salmonella H: g,m Flagellar Antigen and Potential Diagnostic Application


NALBANTSOY A. , KARABOZ İ. , GURHAN I. D.

HYBRIDOMA, vol.29, no.5, pp.419-423, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 5
  • Publication Date: 2010
  • Doi Number: 10.1089/hyb.2010.0023
  • Title of Journal : HYBRIDOMA
  • Page Numbers: pp.419-423

Abstract

In this study, the flagella antigen was detached at high speed by shaking vigorously with glass pearl beads, from Salmonella enteritidis in a yield of 3.9 mg/mL(-1) after being concentrated with polyethylene glycol (PEG). A monoclonal antibody (MAb), designated D7 clone, was generated from Balb/c mice immunized with Salmonella enteritidis flagella using the conventional hybridoma method. The D7 clone secreting MAb was classified as IgG2a isotype. ELISA analyses of D7 MAb to Salmonella-specific flagella demonstrated that the antibody reacted with H: m. However, Western immunoblot analyses of D7 clone appear to be secreting heavy chain of IgG2a antibody, which was eligible for the diagnosis of Salmonella enteritidis.