Zinc and copper contents in the edible parts (muscle, fillet) of 49 commercially used fish species from the eastern Mediterranean Sea (Izmir Outer Bay, Homa Lagoon/Izmir and Mersin Bay) were determined by differential pulse stripping anodic voltammetry. In the sample preparation step, all frozen fish samples were lyophilized, milled in a ball mill and finally decomposed in an oxygen plasma ashing chamber. Among the three sampling locations, the highest zinc concentration was found in Mediterranean shad (Alosa fallax nilotica) from Izmir Outer Bay with 9.73 mg/kg wet weight and the highest copper content was found in derbio (Trachinotus ovatus) from Homa Lagoon with 1.14 mg/kg wet weight. The accuracy of the concentrations determined in this study was checked by the measurements of certified reference material no. 422 cod muscle from the Commission of the European Communities, Community Bureau of Reference. All zinc and copper concentrations observed in species of the eastern Mediterranean Sea showed that fish from this area are good sources of these essential elements.