Determination of Extractability of Pine Bark Using Supercritical CO2 Extraction and Different Solvents: Optimization and Prediction


Yesil-Celiktas O. , OTTO F., Gruener S., PARLAR H.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.57, no.2, pp.341-347, 2009 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 2
  • Publication Date: 2009
  • Doi Number: 10.1021/jf8026414
  • Title of Journal : JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Page Numbers: pp.341-347
  • Keywords: Pine bark, supercritical CO2 extraction, catechins, polarity, optimization, pilot scale, CARBON-DIOXIDE EXTRACTION, PRESSURE SOLUBILITY DATA, GREEN TEA, ANTIOXIDANT ACTIVITY, CATECHINS, PROCYANIDINS, PINASTER, RADIATA, TANNINS, PHASE

Abstract

Bark from Pinus brutia was extracted with Supercritical fluid extraction (SFE), using CO2, at various extraction conditions both at laboratory and at pilot scale. Optimized parameters were 200 bar, 60 degrees C, and 3% ethanol at a solvent/feed ratio of 30. Additionally, the pine bark was sonicated (1 h at 50 degrees C) by different solvents (n-hexane, dichloromethane, ethyl acetate, and ethanol) to investigate the correlation between the different extraction setups and to obtain information on SFE up-scaling possibilities. Analyzed by HPLC, 7.2% of (-)-catechin was extractable at laboratory scale, and 58.4% (800 bar) and 47.8% (200 bar), both with modifiers, at pilot scale. By sonication with ethanol, 46.8% of (-)-catechin and almost 100% of (-)-epicatechin and (-)-catechin gallate were extracted. Ethyl acetate extract revealed high correlations with the laboratory scale SFE (r = 0.98) and also pilot scale SFE runs at 200 (r = 0.99) and 800 bar (r = 0.98) without modifiers.