Doxycycline down-regulates matrix metalloproteinase expression and inhibits NF-kappa B signaling in LPS-induced PC3 cells


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ÖĞÜT D., REEL B. , KORKMAZ C. G. , ARUN M. Z. , Micili S. C. , Ergur B. U.

FOLIA HISTOCHEMICA ET CYTOBIOLOGICA, vol.54, no.4, pp.171-180, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 54 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.5603/fhc.a2016.0022
  • Title of Journal : FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
  • Page Numbers: pp.171-180
  • Keywords: prostate cancer, PC3 cells, MMPs, doxycycline, NF-kappa B, immunohistochemistry, Western blotting, zymography, IN-VITRO MODULATION, PROSTATE-CANCER, INVASION, MIGRATION, TETRACYCLINES, SUPPRESSION, CYTOKINES, LINES, MMP-9, MAPK

Abstract

Introduction. Matrix metalloproteinase enzymes (MMPs) play important role in inflammation, malignant cell proliferation, invasion and angiogenesis by mediating extracellular matrix degradation. Doxycycline, a synthetic tetracycline, behaves as a MMP inhibitor at a subantimicrobial dose and inhibits tumor cell proliferation, invasion and angiogenesis. The aberrant activity of nuclear factor kappa B (NF-kappa B) causes activation of MMPs and thereby proliferation and invasion of cancer cells. The aim of this study was to investigate the effects of doxycycline on the expression of MMPs in lipopolysaccharide (LPS)-induced PC3 human prostate cancer cells and the possible role of NF-kappa B signaling.