Voltammetric and Impedimetric Detection of Interaction Between Dacarbazine and Nucleic Acids


Eksin E., Polat D., Erdem A.

ELECTROANALYSIS, vol.31, no.10, pp.2012-2019, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 10
  • Publication Date: 2019
  • Doi Number: 10.1002/elan.201900284
  • Title of Journal : ELECTROANALYSIS
  • Page Numbers: pp.2012-2019
  • Keywords: DNA-drug interaction, Electrochemical biosensor, Dacarbazine, Differential pulse voltammetry, Electrochemical impedance spectroscopy, ANTICANCER DRUG DACARBAZINE, ELECTROCHEMICAL DETECTION, DNA HYBRIDIZATION, CARBON NANOTUBES, SINGLE, BINDING, BLEOMYCIN, COMPLEX

Abstract

Dacarbazine (DTIC) is a chemotherapy drug that is used for the treatment of Hodgkin's lymphoma, malignant melanoma, childhood solid tumors and soft tissue sarcoma. The surface confined interaction between DTIC and nucleic acids was investigated for the first time in this study by using both differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) in combination with disposable pencil graphite electrodes. The oxidation signals of DTIC and guanine were measured before and after interaction process using DPV technique. The interaction of DTIC with nucleic acids; poly[A], poly[G], or double stranded of poly[A]-poly[T] and poly[G]-poly[C] was also examined using DPV. Furthermore, EIS technique was utilized for detection of the interaction between DTIC and nucleic acids; ssDNA/dsDNA, poly[A], poly[G], or double stranded poly[A]-poly[T] and poly[G]-poly[C].