Characterisation of in vitro gastrointestinal digests from low fat caprine kefir enriched with inulin

Şimşek Ş. , Sanchez-Rivera L., Nehir E. S. , Karakaya S. , Recio I.

INTERNATIONAL DAIRY JOURNAL, vol.75, pp.68-74, 2017 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 75
  • Publication Date: 2017
  • Doi Number: 10.1016/j.idairyj.2017.07.004
  • Page Numbers: pp.68-74


In vitro gastrointestinal digests from low fat caprines' milk kefir were characterised. The impact of the addition of different types of inulin (native, short-chain and long-chain) as a fat replacer on the subsequent release of peptides during digestion was also studied. A total of 52 peptides were identified, mainly from b-casein, following digestion. The gastrointestinal digests possessed angiotensin-converting enzyme (ACE)-inhibitory activity with IC50 values ranging from 190.71 +/- 2.91 to 364.68 +/- 31.27 mu g protein mL(-1). Among the identified peptides, four of them have been previously described as ACEinhibitors (LHLPLP, HLPLP, DKIHP, MAIPPK), and the presence of these sequences can explain the moderate ACE-inhibitory activity found in the samples. Rheological analysis revealed that all manufactured kefirs exhibited shear-thinning behaviour. No significant effect of viscosity on protein degradation during simulated gastrointestinal digestion was found under the working conditions. (C) 2017 Elsevier Ltd. All rights reserved.