In this study, the spawning biomass of the European Anchovy Engraulis encrasicolus (Linnaeus, 1758) found in Edremit Bay in the Aegean Sea was estimated using the Daily Egg Production Method (DEPM). The study was conducted in July 2010. For the determination of the daily egg production (P-0), the eggs of the European Anchovy were collected with a WP2 model plankton net at 23 predetermined stations in the bay. In order to determine the batch fecundity and the spawning frequency, adult fish specimens were caught with boat-seine and trawl boats. The biomass parameters were calculated as follows: daily egg production (P-0): 36.52 eggs/m(2), average weight of mature females (W): 10.78 g, sex ratio (R): 0.61, batch fecundity (F): 4345 eggs, spawning frequency (S): 0.18. Consequently, the stock biomass of the European Anchovy found in Edremit Bay was calculated to be 403.9 MT.